GBXr.comGrain Genes : gbxRMapping of RFLP probes characterized for their polymorphism on wheat. Abstract: Wheat anonymous probes were selected for their efficiency for providing a readable hybridization pattern and revealing RFLP among wheat varieties. We report the mapping of 132 such probes (20 wheat-leaf cDNA, 28 wheat-root cDNA and 84 genomic DNA) on the reference population of the International Triticeae Mapping Initiative (ITMI) derived from the cross W-7984 with Opata85. Each probe has been characterized for its polymorphism information content. The 132 probes allowed us to map 160 loci. RFLP · Wheat · Genetic mappingThis article is referenced by 5 newer articles...
Genetic mapping.Developing new and better tools to make gene hunts faster, cheaper and practical for any scientist was a primary goal of the Human Genome Project (HGP). One of these tools is genetic mapping, the first step in isolating a gene. Genetic mapping - also called linkage mapping - can offer firm evidence that a disease transmitted from parent to child is linked to one or more genes. It also provides clues about which chromosome contains the gene and precisely where it lies on that chromosome. Genetic maps have been used successfully to find the single gene responsible for relatively rare inherited disorders, like cystic fibrosis and muscular dystrophy. Maps have also become useful in guiding scientists to the many genes that are believed to interact to bring about more common disorders, such as asthma, heart disease, diabetes, cancer and psychiatric conditions. Restriction fragment length polymorphismRFLP- In molecular biology, the term restriction fragment length polymorphism (or RFLP) is used in two related contexts: as a characteristic of DNA molecules (arising from their differing nucleotide sequences) by which they may be distinguished, and as the laboratory technique which uses this characteristic to compare DNA molecules. The technique is utilized in genetic fingerprinting and paternity testing.
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